Anesthetization is confirmed when the mouse ceases voluntary movement and has been immobile for over 30 s. You may start to sweat.
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Mouse body temperature measurement. Thermocouples thermistors and rtds. At present mouse body temperature data for metabolic phenotyping is mainly obtained by one of three methods. Abdominal temperature was measured using. The body is very good at keeping its temperature within a safe range even when temperatures outside the body change a lot. When you are too hot the blood vessels in your skin widen to carry the excess heat to your skins surface. Temperatures at ranges between 2 and 150 mm.
The core body temperature of mice has been measured by either the insertion of a thermometer into the anus of the mouse or insertion of a thermocouple via the anus into the large intestine soothilletal1992wong etal1997. In fact the temperature measured by this method is one of highest temperatures in the body of endotherms corresponding to deep core body temperature donhoffer 1980. Body temperature is a measure of your bodys ability to make and get rid of heat. We show that body surface temperatures are about 20 c lower than rectal probe measurements but the. 2 measuring temperature in the abdominal. When precise temperature measurements are required wpi can provide you with a very accurate monitor and thermocouple microprobes.
The measuringdevice is then left insituuntil the temperature recorded stabilizes. Anesthetize by using 1 lmin flow of oxygen with 5 isoflurane. Among studies involving core and surface temperature measurements in mice the surface temperature is on average 257 c 34 c or 23 c lower than core temperature depending on the site of. Wpi monitors have both resolution and accuracy of 01c in the 0 50c range and are traceable to nist standards whereas other competitive electronic thermometers have an accuracy that is usually to 05c or worse. In adult mice an insertion depth of 2 cm will yield colonic temperatures. 1 inserting a probe into the rectum or less typically into the sigmoid colon.
This method of body temperature measurement is successful in reliably replicating temperature change trends during passive system anaphylaxis experiments in mice. Mouse body temperature measurement during anesthetization. Hi karin you could use passive infrared transponder pit tags that include a temperature sensor injected under the skin to measure subcutaneous body temperature a couple of companies make these. Each measuring procedure can. Place the mouse in an anesthesia induction box. Twenty four hour brown adipose tissue bat thermometry and abdominal body temperature readings in a freely moving c57bl6 mouse maintained at 26 c.
Gallery of Mouse Body Temperature Measurement
